The rapid analysis provided by the Large Area Scan Macroscope with a resolution of 5 μm has proven to be an essential tool for inspecting the microstructures of e.g. ice cores in the laboratory.
Scanner head
Time-consuming inspection using a microscope can be replaced by using the specially developed Large Area Scan Macroscope (LASM) with a monochrome Line Scan Camera.The Large Area Scan Macroscope consists of a Line Scan Camera, a high resolution lens as well as an illumination unit. The sample is imaged in reflection with a resolution of 5 μm (5080 dpi). The scanner has three motorized axes. The measuring width is 41 mm per pass with a maximum scan length of 600 mm. A second motorized axis allows up to 100 mm scan width by scanning the sample with three passes. The third motorized axis allows a comfortable focus setting on the image plane.
Bright-field illumination
In order to capture the relevant microstructures, brightfield illumination is used. The light directed at the sample is reflected by surfaces parallel to the sensor. Light reflected from structured areas and edges is reflected away from the sensor and appears dark. Thus, also in the images obtained with this method, the grain boundaries appear as dark lines and gas inclusions appear as dark bubbles or spots.
Stable design with extended scan length
The scanner system is mounted on a granite plane that makes sure that the high resolution of 5 µm is accomplished for the complete, large scan width of 600 mm.
Undisturbed, High Quality Images in Much Less Time
While for the image acquisition technique using a conventional microscope, thousands of images have to be stitched to form a complete picture, only two or three scans are necessary using the Large Area Scan Macroscope depending on sample dimensions. This reduces the imaging time considerably and obviates the alignment and matching of the many individual images of these sections, which requires significant computing time. Since the microscope method takes a long time, for scanning ice cores, all images are additionally taken with slightly different contrast due to the ongoing sublimation process, which also needs to be corrected for. In order to stitch the complete picture, the images also have to be corrected for vignetting and distortion. Using the Large Area Scan Microscope, a shading correction done prior to scanning allows for evenly illuminated images that also do not show significant signs of distortion due to an excellent correction of the field of curvature. Since only two or three images are necessary to cover the whole sample the time required for stitching is severely reduced.
One of many applications: Ice core inspection
The figure on the right shows the 600 mm scan of a longitudinal cut ice core sample.The grain boundaries (dark lines) and pores from different time periodes can be analyzed in one scan. The scan takes about 6 minutes.
A stratigraphic image that supports dating the ice cores can be obtained using the Intermediate Layer Core Scanner (ILCS).
High resolution imaging in harsh environments
As the analysis of the microstructure needs to be done in the lab at low temperatures, the line scanners developped fo analyzing ice cores need to be stabile and insensitive to the harsh environment. The components used (mechanical, optical as well as electrical) are designed to work properly at temperatures down to -20°C. They are used in the lab, e.g. at AWI in Bremerhaven.
More information on microstructure mapping of ice cores can be found on https://www.awi.de/forschung/geowissenschaften/glaziologie/werkzeuge/microstructure-mapping.html.
DEEPICE Project
http://pastglobalchanges.org/science/end-aff/deepice
